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. 2017 May 2;7:1342. doi: 10.1038/s41598-017-01386-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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B Cells internalize older EVs more readily. (A) EV plasma samples (n = 62; 2.00^E+08 particles) from young, middle-aged and older individuals were incubated with 1 old (1) and 1 young (2) PBMC recipient. (B) In a separate experiment, EV plasma samples (n = 57) from young, middle-aged and older individuals were incubated with a different old (3) and young (4) PBMC recipient. In both experiments, EVs were incubated with PBMCs for 24 hours and then anayzed by FACS. The points are the actual data (log transformed) and the line is from the model accounting for matching. (C) CD25 Mean Fluorescence Intensity (MFI) in B cells that interacted with EVs (CD19⁺PKH⁺CD25⁺) was plotted against the EV donor age. (D) MHC-II MFI in monocytes that interacted with EVs (CD11b⁺PKH⁺MHC-II⁺) was plotted against EV donor age. The graphs in C-D are 57 EV donors that were incubated with PBMC recipient 3. For all graphs, linear mixed model regression was used to analyze the relationships accounting for both sex and race matching and P values are indicated.