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. 2017 Apr 25;7:1325. doi: 10.1038/s41598-017-01256-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HepaChip: (A) Full view of the chip with 8 culture chambers, fluidic inlet and outlet and gold electrodes. (B) close up of one chamber containing 2 electrodes and 3 assembly ridges coated with collagen. (C) Simulation of flow velocity and trajectories of cells during DEP assembly inside a culture chamber. (D) PANC1 cells assembled on one assembly ridge right after assembly. (E) Live/Dead staining of PANC1 cells after 146 hours of perfused culture inside the HepaChip® chamber.