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. 2017 Apr 25;7:1325. doi: 10.1038/s41598-017-01256-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro cell growth characteristics of PDAC cells in the HepaChip^®. (A) Live/Dead cell staining of BxPC3: BxPC3 grow selectively on the ridge. (B) Live/Dead cell staining of PANC1: In contrast the PANC1 spread as well channel walls and bottom, so the picture on day 6 was taken in two focal planes: on the channel and on the ridge. (C) Mitosis of BxPC3: Mitosis was observed inside the chip on the collagen coated area after 16 h culture. From left to right pictures taken after 0 min – 1 min – 4 min – 16 min using an inverted microscope. (D) Cytoskeleton assembly of BxPC3 and (E) of PANC1: Fluorescence images after actin and nucleus staining with Phalloidin and DAPI. Scale bars in A and B represent 100 µm, in C represents 10 µm.