Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 25;7:1325. doi: 10.1038/s41598-017-01256-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of cisplatin on PANC1 cells. (A) Dose response curve of Cisplatin on PANC1 cells in 2D culture conditions. The calculated IC₅₀ was 3.25 ± 0.2 μM. Data are means ± SD of two separate experiments, each carried out in triplicate. (B) Dose response curve of Cisplatin on PANC1 cells in 3D culture conditions. The calculated IC₅₀ was 14.6 ± 1.6 μM. Data are means ± SD of two separate experiments, each carried out in triplicate. Panels on the right show the live/dead cell staining of the spheroids in control (panel on the top), 25 μM (panel in the middle), and 100 μM (panel at the bottom). (C) Effect of cisplatin on PANC1 cells subcutaneously injected into mice (in vivo). The trend line shows the volume of the masses during the duration of experiment, histogram on the right shows the tumour masses of explant from the animals. (D) Effect of cisplatin on PANC1 cells inside the HepaChip^® under continuous perfusion of 3 µL/min. Live/Dead cell staining after 72 h incubation with 0 µM, 25 µM and 100 µM cisplatin; inset: black framed area of the chip cell culture before incubation with 100 µM Cisplatin. Scale bars 100 µm. (E) Effect of different cisplatin concentrations on PANC1 cells cultured inside the HepaChip^®.