Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 2;7:1347. doi: 10.1038/s41598-017-00758-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Multiplexed paper devices: performing several simultaneous reactions thanks to liquid flows. (a) Picture of an unfolded wax geometry (multilayered geometry), with dry amounts of dyes in the second last layer. Picture of the same device after folding and dipping it in water: the dyes are conveyed to the top without mixing together. (b) Schematics of the unfolded multilayered wax geometry and location of freeze-dried RT-RPA reagents and targeted RNA template. Exploded view of the folded device: the paper tape should be dipped into water and sample pipetted in the sample test outlet. (c) Results of amplification (final intensity value) for each set of primers (A, B or C) with or without the related RNA template in the sample test outlet (grey bars), with associated controls (positive: black bars; negative: white bars). (d) Picture of an unfolded longer wax geometry (multiplexed device), with dry amounts of dyes in the second last layer. Picture of the same device after folding and dipping into water: the dyes are conveyed to the top without mixing together. Picture of the same device, without dry components stored in it, fluorescein is pipetted in the middle spot simultaneously to the strip dipped in water. The yellow dye is distributed towards three sample tests outlets. (e) Schematics of the multiplexed paper device: exploded view and top view, and location of freeze-dried biological components (RT-RPA reagents with three different primers and associated RNA templates). (f) Normalized compared results of specific amplification of each template in the 9-outlet device (positive controls: black bars, negative controls: white bars; sample tests: grey bars).