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. 2017 May 2;7:1314. doi: 10.1038/s41598-017-01462-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of lentinan on expression of cytokine and inflammasome components. (A) BMDMs were treated with the indicated dosage of lentinan (LNT) or LPS (10 ng/mL). Expression of IL-1α, TNFα, IL-6, IL-1rn, IL-10, Pro-IL-1β, NLRP3, NLRC4, AIM2, and Pro-Casp1 mRNAs was measured by RT-PCR and the right bar graph indicates relative band density. (B) BMDMs were treated with LPS (10 ng/mL) or LNT (1 mg/mL) with/without TAK-242, TLR4 inhibitor. Indicated gene expression levels were assessed by RT-PCR. (C) BMDMs were treated with LNT purified by endotoxin removal resin (Endotoxin-removed LNT) or intact LNT as indicated. NLRP3 and Pro-IL-1β proteins were measured by immunoblotting. (D) LPS-primed BMDMs were treated with endotoxin-removed LNT with/without dsDNA. Secretion of IL-1β was measured by ELISA. All RT-PCR data shown are representative of at least three independent experiments. Bar graph presents the mean ± SD.