Figure 3.

Effect of 18-carbon fatty acid on Nrf2 and CYP2A6 expression in HepG2 cells. (A) HepG2 cells viability which were administered with increasing concentrations of SA, OA, LA, and ALA. The cells viability was represented by the absorbance. The effect of SA, OA, LA, and ALA on Nrf2 and CYP2A6 protein expression in HepG2 cells are exhibited in panel (B). Nrf2 protein expression was quantified and shown in panel (C). CYP2A6 protein expression was quantified and shown in panel (D). The effect of SA, OA, LA, and ALA on Nrf2 and CYP2A6 mRNA expressions are shown in panel (E,F), respectively. Asterisks ^* represent statistical difference caused by fatty acid administration from control cells. ^* 0.01< P < 0.05, ^**P < 0.01.