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. 2017 May 15;8:233. doi: 10.3389/fphar.2017.00233

Figure 4.

Figure 4

Nrf2 gene silence and over expression. In Nrf2 siRNA transfected HepG2 cells, Nrf2 protein expression is shown in panel (A) and its quantity relative to β-actin is shown in panel (B); Nrf2 mRNA expression is shown in panel (C). In pcDNA3-EGFP-C4-Nrf2 transfected HepG2 cells, Nrf2 protein expression is shown in panel (D) and its quantity relative to β-actin is shown in panel (E); Nrf2 mRNA expression is shown in panel (F). The GSTA1 mRNA relative expression in different groups was shown in panel (G). NC, in panel (A–C) represents the HepG2 cells that transfected with negative control siRNA, in panel (D–F) represents the HepG2 cells that transfected with negative control plasmid (pcDNA3-EGFP-C4). In panel (G), NC-O represents the cells that transfected with pcDNA3-EGFP-C4 and NC-S represents the HepG2 cells that transfected with negative control siRNA. Cell, means the control cells that without any administration. Asterisks * represent statistical difference caused by Nrf2 siRNA or pcDNA3-EGFP-C4-Nrf2 transfection from control cells without any stimulation. * 0.01< P < 0.05, **P < 0.01.