Figure 5.

CYP2A6 and GSTA1 expression changes in Nrf2 silenced and over-expressed HepG2 cells administered with LA and ALA. CYP2A6 mRNA (A) and protein (B,C) expressions were both decreased significantly in Nrf2 silenced cells with or without LA and ALA treatment. CYP2A6 mRNA (D) and protein (E,F) expressions were increased in Nrf2 over-expressed cells with or without LA and ALA administration. In the presence or absence of LA and ALA administration, GSTA1 mRNA expression was down-regulated in cells transfected with Nrf2 siRNA (G) and was up-regulated in cells transfected with pcDNA3-EGFP-C4-Nrf2 (H). In addition, the NC cells in panel (A–C,G) were transfected with NC siRNA. The NC cells in panel (D–F,H) were transfected with NC plasmid (pcDNA3-EGFP-C4). ^* represent statistical difference caused by transfection of Nrf2 siRNA and pcDNA3-EGFP-C4-Nrf2 from cells transfected with NC siRNA and NC plasmid; $ represent statistical difference caused by LA and ALA administration within Nrf2 silenced cells, Nrf2 over expressed cells, and control cells. ^*0.01 < P < 0.05, ^**P < 0.01; $ 0.01 < P < 0.05, $$P < 0.01.