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. 2017 May 2;7:1297. doi: 10.1038/s41598-017-01272-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Triacylglycerol (TAG) and its unsaturation degree (N _C=C) in the sirloin of Japanese Black cattle. (A) ¹H NMR spectra of the CDCl₃ extract of a sirloin sample after 2-week aging. Each signal is assigned to the proton represented by the italic and bold style (H) in the partial structural formula of TAG. (B) The N _C=C values of the sirloin samples from four different brands. The data are presented as the mean ± standard error (SE; n = 5). Means with different letters (a and b) are significantly different at p < 0.05.