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. 2017 May 2;7:1297. doi: 10.1038/s41598-017-01272-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Multivariate data analysis of the ¹H NMR spectra of the D₂O extracts of ribeye samples with different aging duration. Ribeye samples are derived from heifers and delivered cows. (A) PCA score plot of PC1 versus PC2. The plots are color-coded according to aging duration (week): 0, blue; 2, cyan; 4, turquoise; 6, light green; 8, yellow; and 10, red. The heifer samples are positioned separately from the delivered cow on the score plot, and the individual regions are divided by a dashed line. (B) Loading plot for PC1 of the score plot shown in panel A. Buckets with high loading values are labeled by the assigned compound names. The square brackets represent the chemical shift range of each spectral bucket. (C) OPLS regression model for the prediction of aging duration. Predicted values are derived from the OPLS model of the ¹H NMR spectra. The plots are color-coded according to aging duration (week): 0, blue; 2, cyan; 4, turquoise; 6, light green; 8, yellow; and 10, red. (D) S plot for the OPLS regression model shown in panel C. Buckets with high absolute values of p[1] and p(corr)[1] are labeled with the assigned compound names. The square brackets represent the chemical shift range of each spectral bucket.