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. 2017 May 2;7:1327. doi: 10.1038/s41598-017-01455-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of NGF, p75^NTR and TrkA in permanent human teeth after cavity preparation. (A–C) Histology of dental tissues after cavity preparation. (D) Overview of NGF staining (brown colour) in tooth after cavity preparation. (D(a–c)) Higher magnifications of D. (E) p75^NTR expression (brown colour) in newly formed odontoblasts underlying tertiary dentin. (F) p75^NTR expression in nerve fibres in proximity of tertiary dentin. (G) p75^NTR expression in odontoblasts (arrowheads) and in nerve fibres within the pulp. (H,I) TrkA expression (brown colour) in odontoblasts and nerve fibres adjacent to the cavity (H) and far away from the injury site (I). (J) Negative control. Abbreviations: d, dentine; do, dying odontoblasts; f, nerve fibres; no, newly-formed odontoblasts; o, odontoblasts; p, pulp; v, vessel. Asterisk indicates the cavity.