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. 2017 May 2;7:1301. doi: 10.1038/s41598-017-01368-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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UV tolerance and cold stress test. (a) Bar graph quantifying conidial germination exposed AC and MFC of Hirsutella satumaensis to UV radiation for different duration range 0 s to 120 s. UV irradiation slowed the growth of both types of spores, but AC was less affected. Conidial germination was significantly different between AC and MFC (p < 0.05) following UV irradiation for 20 s (37.46 ± 1.372% vs. 32.26 ± 3.862%, respectively), 40 s (28.71 ± 3.831% vs. 23.13 ± 3.592%) or 60 s (17.97 ± 2.881% vs. 13.17 ± 2.380%). (b) Testing the spore viabilities of AC and MFC under cold stress (0 °C, 4 °C and 10 °C). Germination rate of MFC decrease significantly, especially at 0 °C. Statistical analysis was performed using a one-way ANOVA. Error bars are standard deviations of three trials and panels are representative of at least 2 independent experiments, *p < 0.05.