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. Author manuscript; available in PMC: 2018 Mar 8.
Published in final edited form as: J Am Chem Soc. 2017 Feb 21;139(9):3332–3335. doi: 10.1021/jacs.6b12866

Figure 1.

Figure 1

An aspartate-containing substrate is modified by OGT. (a) Sequences of peptides used in this study. The ‘*’ denotes that S10-35 bears 3 N-terminal lysine residues not found in the other peptides. (b) EIC traces for [M+GlcNAc] for both D10-32 (top) and A10-32 (bottom) after 5 min incubation with OGT. (c) EIC traces of 841.6279 [M] (left panel) and 837.1253 [M-H2O] (right panel) for D10-32 show conversion of starting material (S.M.) over time to a species isobaric with starting material (S.M. isomer) and to an anhydropeptide.