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. Author manuscript; available in PMC: 2018 Mar 8.
Published in final edited form as: J Am Chem Soc. 2017 Feb 21;139(9):3332–3335. doi: 10.1021/jacs.6b12866

Figure 4.

Figure 4

OGT converts aspartic acid to isoaspartate. (a) PIMT catalyzes isoaspartate methylation, producing a succinimide that hydrolyzes to a mixture of aspartate and isoaspartate. (b) EIC traces for 841.6279 (4+), corresponding to D10-32 starting material and isomer. Traces are after treatment with OGT alone (top) or with OGT followed by PIMT (bottom). (c) Normalized succinimide abundance for OGT and PIMT treated D10-32 (left), pure isoD10-32 (middle) or pure D10-32 (right); abundances were measured by integrating the respective EIC traces for succinimide, 837.1253 (4+).