Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 2;7:1424. doi: 10.1038/s41598-017-01542-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Global identification of human aminopeptidase N (hAPN) substrate specificity with the MSP-MS assay. (A) IceLogo representation of P1–P4′ specificity at the 60 min assay time point (P ≤ 0.05 for all residues shown; “n” is norleucine). Residues with a positive percent difference are considered favorable at a given position; residues with a negative percent difference are considered disfavorable. (B) Heat map representation of hAPN P1–P4′ specificity at the 60 min assay time point calculated using Z-scores at each position. Favored residues are colored blue (Z-score > 0) and disfavored residues are colored red (Z-score < 0). iceLogo representations and heat maps for the 15, 240, and 1200 min assay time points are provided (Supplementary Figure 1). (C) Example 14-mer peptides from the MSP-MS library are shown with primary and secondary cleavages indicated with a blue arrow. “X” indicates that no cleavage was detected at the indicated position. A progress curve is provided depicting the total cleavages observed at each assay time point.