Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 3;7:1416. doi: 10.1038/s41598-017-01641-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

GATA4/TBX5 interaction is not affected by Thalidomide. Protein-protein docking interface between TBX5 and GATA4 modeled DNA binding domain as retrieved from PDB. Only Residues of TBX5 within 5 angstroms of this interaction are shown (A). The position of thalidomide in the context of the predicted TBX5-GATA4 interaction is clearly away from the interface region (B): TBX5 (cyan) and GATA4 (magenta). The physical interaction between both proteins was assessed by co-immunoprecipitation in the presence or absence (−) of either 4 µM thalidomide or DMSO (C). Ten times the quantity of proteins loaded for western blot was used for immune-precipitation. Nuclear lysates for both GATA4 and TBX5 were immune-precipitated with anti‐HA antibody and GATA4 proteins were visualized by western blot with an anti‐flag antibody. Membrane stripping and subsequent western blot analysis was performed with anti‐HA antibody in order to detect TBX5 proteins. Quantitation of the bands didn’t show any significant change. (Ctr: control, WB: Western-Blot, IP: Immuno-Precipitation).