Table 1.

Analysis of intracellular pools of pathway intermediates.

Strain	Concentrations after 48 h (mg/L)
	L-Phenylalanine	Cinnamic acid	p-Coumaric acid	Pinosylvin
pCDFD-T7-RgPALa	99 ± 3	249 ± 4	27 ± 0.4	0
pCDFD-T7-TcPALb	125 ± 3	465 ± 6	40 ± 0.3	0
pCDFD-Trc-RgPAL-Trc-4CLc	90 ± 2	200 ± 3	26 ± 0.3	3.7 ± 0.03
pCDFD-Trc-TcPAL-Trc-4CLd	116 ± 3	404 ± 6	36 ± 0.2	10.5 ± 0.11

^aRgPAL was directly cloned into NcoI/AvrII sites of pCDFDuet-1. Engineered strains contained pCOLA-T7-aroF^wt-T7-pheA^fbr and pCDFD-T7-RgPAL.

^bTcPAL was directly cloned into NcoI/AvrII sites of pCDFDuet-1. Engineered strains contained pCOLA-T7-aroF^wt-T7-pheA^fbr and pCDFD-T7-TcPAL.

^cEngineered strains contained pCOLA-T7-aroF^wt-T7-pheA^fbr, pCDFD-Trc-RgPAL-Trc-4CL, pETD-T7-STS.

^dEngineered strains contained pCOLA-T7-aroF^wt-T7-pheA^fbr, pCDFD-Trc-TcPAL-Trc-4CL, pETD-T7-STS.