Table 3.
Plasmids used in this study.
| Plasmids | Description | Source or reference |
|---|---|---|
| pETDuet-1 | Double T7 promoters, pBR322 ori, AmpR | Novagen |
| pCDFDuet-1 | Double T7 promoters, CloDF13 ori, SmR | Novagen |
| pACYCDuet-1 | Double T7 promoters, P15A ori, CmR | Novagen |
| pRSFDuet-1 | Double T7 promoters, RSF ori, KnR | Novagen |
| pCOLADuet-1 | Double T7 promoters, COLA ori, KnR | Novagen |
| pCDFD-Trc | T7 promoter was replaced by Trc promoter | This study |
| pRSFD-Trc | T7 promoter was replaced by Trc promoter | This study |
| pACYC-Trc | T7 promoter was replaced by Trc promoter | This study |
| pETD-Trc | T7 promoter was replaced by Trc promoter | This study |
| pCDFD-T7-TcPAL | pCDFDuet-1 carrying TcPAL under T7 promoter | This study |
| pCDFD-T7-RgPAL | pCDFDuet-1 carrying RgPAL under T7 promoter | This study |
| pCDFD-Trc-TcPAL | pCDFDuet-1 carrying TcPAL under Trc promoter | This study |
| pACYC-T7-TcPAL | pACYCDuet-1 carrying TcPAL under T7 promoter | This study |
| pCOLA-T7-TcPAL | pCOLADuet-1 carrying TcPAL under T7 promoter | This study |
| pETD-T7-TcPAL | pETDuet-1 carrying TcPAL under T7 promoter | This study |
| pCDFD-Trc-4CL | pCDFDuet-1 carrying 4CL under Trc promoter | This study |
| pCDF-Trc-TcPAL-Trc-4CL | pCDFDuet-1 Carrying TcPAL and 4CL under Trc promoter | This study |
| pCOLA-T7-aroFwt-T7-pheAfbr | pCOLADuet-1 carrying aroF wt and pheA fbr | 16 |
| pCDFD-Trc-aroFwt | pCDFDuet-1 carrying 4CL under Trc promoter | This study |
| pCOLA-Trc-aroFwt-Trc-pheAfbr | pCOLADuet-1 carrying aroF wt and pheA fbr under Trc promoter | This study |
| pCOLA-Trc-aroFwt-Trc-pheAfbr-Trc-TcPAL | pCOLADuet-1 carrying aroF wt, pheA fbr and TcPAL under Trc promoter | This study |
| pACYC-Trc-aroFwt-Trc-pheAfbr-Trc-TcPAL | pACYCDuet-1 carrying aroF wt, pheA fbr and TcPAL under Trc promoter | This study |
| pCDFD-Trc-aroFwt-Trc-pheAfbr-Trc-TcPAL | pCDFDuet-1 carrying aroF wt, pheA fbr and TcPAL under Trc promoter | This study |
| pETD-Trc-aroFwt-Trc-pheAfbr-Trc-TcPAL | pETDuet-1 carrying aroF wt, pheA fbr and TcPAL under Trc promoter | This study |
| pCDFD-T7-aroFwt-T7-pheAfbr-T7-TcPAL | pCDFDuet-1 carrying aroF wt, pheA fbr and TcPAL under T7 promoter | This study |
| pACYC-T7-aroFwt-T7-pheAfbr-T7-TcPAL | pACYCDuet-1 carrying aroF wt, pheA fbr and TcPAL under T7 promoter | This study |
| pCDFD-Trc-4CL-Trc-STS | pCDFDuet-1 carrying 4CL and STS under Trc promoter | This study |
| pETD-Trc-4CL-Trc-STS | pETDuet-1 carrying 4CL and STS under Trc promoter | This study |
| pACYC-Trc-4CL-Trc-STS | pACYCDuet-1 carrying 4CL and STS under Trc promoter | This study |
| pRSFD-Trc-4CL-Trc-STS | pRSFDuet-1 carrying 4CL and STS under Trc promoter | This study |
| pCDFD-T7-4CL-T7-STS | pCDFDuet-1 carrying 4CL and STS under T7 promoter | This study |
| pACYC-T7-4CL-T7-STS | pACYCDuet-1 carrying 4CL and STS under T7 promoter | This study |
| pRSFD-T7-4CL-T7-STS | pRSFDuet-1 carrying 4CL and STS under T7 promoter | This study |
| pET-T7-4CL-T7-STS | pETDuet-1 carrying 4CL and STS under T7 promoter | This study |
| pETD-T7-4CL(N)-T7-STSa | T7 promoter was replaced by Trc promoter | This study |
| pETD-T7-4CL(−5.0)-T7-STS(M)b | pCDFDuet-1 carrying STS | This study |
a N = −10.3, −9.4, −8.1, −7.6, −7.0, −5.0, −4.1.
b M = −8.0, −6.3, −5.1, −4.9, −4.4.