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. 2017 May 3;7:1437. doi: 10.1038/s41598-017-01440-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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fdGOGAT assay with complete and incomplete reaction mix. S. elongatus cells were grown to an OD₇₅₀ of 0.3 in nitrate-supplemented medium. The 2-OG concentrations in the reaction mixture was determined by FRET over time. Results are shown for a complete reaction mix (black squares and black dashed line) and the blank mix lacking glutamine (white squares and red dashed line). Error bars: ±s.e.m., n = 3.