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. 2017 May 3;7:1437. doi: 10.1038/s41598-017-01440-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of TCA-cycle metabolites on the FRET signal of TC3-R9P in cultured human cells. (A–D) Various concentrations of the compounds were added to the cell culture media of U87-MG glioblastoma cancer cells at time point zero. 10 to 50 cells were evaluated in each experiment. Average values ± s.e.m. are shown. (A) 2-OG analogue dm2-OG; (B) Citrate; (C) Glutamine; (D) Glutamine following pre-incubation with 100 µM of the glutaminolysis inhibitor epigallocatechin gallate (EGCG). (E) Embryonic kidney cells (HEK293T) treated with dm2-OG. (F) Retinal pigment epithelium cells (RPE1) treated with dm2-OG.