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. 2017 May 3;7:1434. doi: 10.1038/s41598-017-01609-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of antioxidants on spindle morphology and chromosome alignment in oocytes at MII stage after pre-maturation aging and IVM. (a) Shows an oocyte with a normal spindle and chromosome alignment. (b) Shows an oocyte with an abnormal spindle and chromosome alignment. Green: a-Tubulin; Red: chromosomes. (Bar = 10 µm). (c) Shows percentages of oocytes with abnormal spindle and/or misaligned chromosomes in oocytes after 12 h pre-maturation aging and IVM. (d) Shows percentages of oocytes with abnormal spindle and/or misaligned chromosomes in oocytes after 24 h pre-maturation aging and IVM. (e) Shows percentages of oocytes with abnormal spindle and/or misaligned chromosomes in oocytes after 36 h pre-maturation aging and IVM. *P < 0.05; **P < 0.01; ***P < 0.001. No. of oocytes at each group was shown in the bracket. AO: antioxidants.