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. 2016 Dec 20;21(6):1206–1216. doi: 10.1111/jcmm.13054

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© 2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Upper panels: confocal immunofluorescence double staining for KIT (green) and MCT (red) showing co‐occurrence of both antigens on a mast cell in the detrusor of rat bladder (yellow arrow). Mast cells are very rare in rat bladder. Gut tissue is used as external tissue control, showing KIT ⁺/MCT ⁺ mast cells (yellow arrows) in the tunica mucosa (TM). Lower panels: confocal immunofluorescence double staining for KIT (green) and VIM (red) showing many VIM ⁺/KIT ⁻ IC (white arrows) in rat bladder. Gut tissue is used as external tissue control, with the presence of KIT ⁺/VIM ⁺ ICC (yellow arrows) and KIT ⁻/VIM ⁺ IC (white arrow) in the tunica muscularis externa (TME). Bl, bladder; LP,lamina propria; D, detrusor; TM,tunica mucosa; TME, tunica muscularis externa. DAPI blue stain illustrates nuclei. Scale bars: 25 μm.