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. 2017 May 3;7:1449. doi: 10.1038/s41598-017-01202-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tissue proteomic analysis of SVZ+ (n = 6) and SVZ- (n = 6) GBMs using 2D-DIGE method. Cy3 and Cy5 overlapped 2D-DIGE gel image of SVZ+ and SVZ- GBM brain tumor tissue proteome and the 3D-views for the significantly altered proteins. The tissue proteins were separated based on their pI using IPG strips of pH 4–7; 18 cm length, followed by separation based on molecular weights using 12.5% SDS-polyacrylamide gels and image analysis was performed using DeCyder 2D software. Significantly altered protein spots were subjected to MALDI-TOF/TOF analysis to establish their identity. Dot plots and 3D-views represent the relative abundance of significantly altered proteins in the SVZ+ and SVZ- GBMs (Green colour indicates Cy3 labeled protein and red colour indicates Cy5 labeled protein).