Skip to main content
. 2016 Dec 13;21(6):1128–1138. doi: 10.1111/jcmm.13044

Figure 2.

Figure 2

Lung histology and analysis of lung morphology in 28‐day‐old pups after perinatal LPS and post‐natal hyperoxia at 65%. At day 14 of gestation, pregnant dams were injected intraperitoneally with 150 μg/kg LPS. After delivery, newborn pups were exposed to 21% O2 (room air) or 65% O2 (hyperoxia) and received daily s.c. injections of either vehicle, 10 mg/kg IL‐1Ra or 100 mg/kg IL‐1Ra from day 1 to day 28. Another group received daily s.c. injections of vehicle from day 1 to day 5 followed by s.c. injections of 10 mg/kg IL‐1Ra from day 6 to day 28 (late onset). For all pups, lung morphology was assessed at day 28, n = 5–27 per group. (A) One representative image per group is shown. Scale bars 100 μm, ×200 magnification. (B‐D) Scans of whole lungs were analysed for alveolar size in μm2 (B), number of alveoli per mm2 (C) and the surface area‐to‐volume ratio (μm2/μm3) (D). Data are shown as mean ± S.E.M. ***P < 0.001 for air vehicle versus hyperoxia vehicle; #P < 0.05 and ##P < 0.01 for hyperoxia vehicle versus hyperoxia 10 mg/kg IL‐1Ra. LPS: lipopolysaccharide, IL‐1Ra: interleukin‐1 receptor agonist.