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. 2017 Feb 9;21(6):1193–1205. doi: 10.1111/jcmm.13052

Figure 4.

Figure 4

Effect of Sestrin 1 overexpression on autophagy. Cardiomyocytes were treated with or without PE and co‐cultured with or without CQ for 12 hrs. (A) The representative immunoblot illustrates the alterations in the levels of LC3BII/GAPDH. (B) Quantitative analysis of data shown in (A). (C) The representative immunoblots illustrates the alterations in the levels of p62 and cathepsin D. Quantitative analysis of levels of p62 (D) and cathepsin D (E). GAPDH was used as an internal control; *P < 0.05 versus the corresponding control group without PE, # P < 0.05 versus Ad‐GFP+PE group. (F) Ultrastructural images captured using TEM show the changes in the number of autophagosomes and autolysosomes. Each of the experiments was repeated five times, n = 5.