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. 2017 Feb 9;21(6):1193–1205. doi: 10.1111/jcmm.13052

Figure 5.

Figure 5

Autophagy blockade weakened antihypertrophic effect of Sestrin 1 overexpression. (A) The knockdown effects of siRNA transfection on Atg7 protein expression and autophagy blockade were evaluated. Representative immunoblots of Atg7, LC3BII and GAPDH levels were shown. (B) Thirty‐six hours after seeding, the cardiomyocytes were transfected with Atg7‐specific siRNA (50 nmol/l) and scramble siRNA (50 nmol/l) in serum‐free medium for 12 hrs. After an additional 6 hrs of adenoviral transfection with Ad‐Sestrin 1 and Ad‐GFP and serum deprivation, cardiomyocytes were treated with PE for 24 hrs. q‐PCR was performed to determine the relative levels of ANP. GADPH was used as an internal control. *P < 0.05 versus scramble+Ad‐GFP+PE group, # P < 0.05 versus corresponding scramble+PE group. Each of the experiments was repeated five times, n = 5.