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. 2017 Feb 9;21(6):1193–1205. doi: 10.1111/jcmm.13052

Figure 6.

Figure 6

Effect of Sestrin 1 knockdown and overexpression on AMPK/mTOR pathway. (A) Representative immunoblots of phosphor‐AMPK, phosphor‐mTOR, phosphor‐p70S6K, phosphor‐Akt (Ser473), AMPK, vinculin, p70S6K and Akt in cardiomyocytes with Sestrin 1 knockdown. (B, C, D, E). Quantitative analysis of phosphorylation of AMPK, mTOR, p70S6K and Akt (Ser473) in cardiomyocytes with Sestrin 1 knockdown. (F) Representative immunoblots of phosphor‐AMPK, phosphor‐mTOR, phosphor‐p70S6K, phosphor‐Akt (Ser473), AMPK, vinculin, p70S6K and Akt in cardiomyocytes with Sestrin 1 overexpression. (G, H, I, J) Quantitative analysis of phosphorylation of AMPK, mTOR, p70S6K and Akt (Ser473) in cardiomyocytes with Sestrin 1 overexpression. *P < 0.05 versus the corresponding control group without PE, #P < 0.05 versus the scramble+PE group or Ad‐GFP+PE group. Each of the experiments was repeated three to seven times, n = 3–7.