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. 2017 Mar 17;13(5):3866–3872. doi: 10.3892/ol.2017.5870

Figure 2.

Figure 2.

miR-548c-3p inhibits T98G cell proliferation and migration. (A) An MTS cell proliferation assay was performed 1, 2, 3 and 4 days after transfection of T98G cells with either miR-548c-3p or NC. (B) Colony formation assay of T98G cells transfected with miR-548c-3p or NC. (C) Transwell migration assay of miR-548c-3p- or NC-transfected T98G cells. (D) Wound healing assay of T98G cells at 0, 1 and 3 days after transfection with miR-548c-3p or NC. (E) Flow cytometry assay of T98G cells with miR-548c-3p or NC. (F) Caspase 3/7 activity assay of T98G cells transfected with either NC or miR-548c-3p. Caspase 3/7 activity is relative to that of NC, set as 1. *P<0.05; **P<0.01. miR, microRNA; MTS, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium; NC, negative control scrambled oligonucleotide; OD, optical density.