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. 2017 May 3;7:1388. doi: 10.1038/s41598-017-01410-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stimulus-dependent yjiY expression under nutrient-limiting conditions. E. coli MG1655 mutant ΔyhjX harboring pBBR yjiY-lux was cultivated in 0.1x LB medium to establish low nutrient conditions. After 1 h (time point 0), the indicated concentration of pyruvate (A), or L-serine (B), or the equivalent volume of water was added. Experiments were performed at least three times (standard deviation <10%), and results of a representative experiment are shown.