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. 2017 May 3;7:1417. doi: 10.1038/s41598-017-01649-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IVPA-US imaging of human coronary atherosclerosis at 16 fps with comparison to histopathology. (a) Picture of collected human heart. (b) Scenario picture of ex vivo IVPA-US imaging of dissected human coronary artery. The region of interest was marked by metal pin. The catheter and sheath were inserted into the artery lumen. Cross-sectional (c) IVPA, (d) IVUS, and (e) merged images of human coronary artery at the region of interest. (f) Gold-standard histopathology stained with Movat’s pentachrome at the region of interest. (g,h) Magnified images of lipid deposition sites corresponding to the dashed boxes in (f). *Indicates the accumulation of cholesterol clefts.