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. 2017 May 3;7:1477. doi: 10.1038/s41598-017-01550-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MTC data showing the effect of miRNA- and anti-miRNA-139-5p. (A) Baseline cell stiffness of individual IAS SMCs before and after 72 h transfection with ROCK2 siRNA, miRNA-139-5p, anti-miRNA-139-5p (inhibitor), and miRNA-139-5p plus the inhibitor. SMCs transfected with ROCK2 siRNA and miRNA-139-p showed decreases in baseline cell stiffness (basal tone) while cells transfected with anti-miRNA-139-5p showed increases in basal tone. Increased basal tone in response to anti-miRNA-139-5p was attenuated with co-transfection with miRNA-139-5p. Data are presented as Mean ± s.e.m. (n = 89–149 individual cell measurements). (B) Dynamic changes the stiffness of IAS SMCs in response to 100 nM U46619. Consistent with the rat smooth muscle data, anti-miRNA-139-5p causes faster and greater cell stiffening in response to U46619.