Table 1.
Synthetic oligonucleotides used in this study.
| Primer | Sequence 5′ → 3′ | Position(s) | Purpose |
|---|---|---|---|
| Oligo(dT)18 | TTTTTTTTTTTTTTTTT | Reverse transcription | |
| P70-promoter up | TTCCATGCACTCTAGCTAAG | −304–285 | Amplification of ChHsp70 promoter fragment for DNA-affinity purification and EMSA |
| P70-promoter down | GAATCGTAGTCTAGTTGACTC | +40–+60 | |
| HP1BP3-up | ACATATTTGAATGTATGTATGAAT | −14–+10 | Amplification of ChHp1bp3 coding sequence |
| HP1BP3-down | ACTGTTGTGGATAAAGATTCCCT | +1528–+1550 | |
| UP | CGGCAGTGGTATCAACGCAGAGTAC | Universal primer used with 3GSP2 and 5GSP2 | |
| UP(dG) | CGGCAGTGGTATCAACGCAGAGTAC(G)10 | 5′ RACE PCR | |
| 5GSP1 | TATTTCTTCATCATTAGCA | +594–+612 | |
| 5GSP2 | TTCCCATGTTTTTCCGCCTCTGAC | +789–+812 | |
| UP (dT) | CGGCAGTGGTATCAACGCAGAGTAC(T)18 | 3′ RACE PCR | |
| 3GSP1 | TACATAGAAAAGTATTAC | +898–+915 | |
| 3GSP2 | TCAGATGACATGTGGCAGGCTGTT | +967–+990 | |
| HP1BP3-F-NdeI | GGGAATTCCATATGTATGAATAT | +1–+12 | Amplification of ChHp1bp3 for protein expression |
| HP1BP3-R-XhoI | CCGCTCGAGTTTTCTGGAGAC | +1420–+1431 | |
| HP1BP3-E-KpnI | ATAAAAGGTACCATGTATGAATATTACACAGAATCCATC | +1–+27 | Amplification of ChHp1bp3 for overexpression |
| HP1BP3-E-XhoI | ATAAAACTCGAGCTATTTTCTGGAGACTGGGGC | +1414–+1434 | |
| Actin-Up | CTGTGCTACGTTGCCCTGGACTT | +701–+723 | RT-PCR |
| Actin-Down | TGGGCACCTGAATCGCTCGTT | +829–+849 | |
| RT-HP1BP3-Up | AAACAGCCTCAGGACAGATG | +1094–+1113 | RT-PCR |
| RT-HP1BP3-Down | CAAGTTGAAATGATCCGCCAC | +1238–+1258 | |
| RT-HSP70-Up | GCCAAACTACATCAGAACGGGTC | −1860–1838 | RT-PCR |
| RT-HSP70-Down | TCCATCTCCTCTACAGTCGGTCC | −2461–2439 |