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Apoptosis of TE-1 cells in each group as detected by flow cytometry. (A) SATB1-siRNA-1; (B) SATB1-siRNA-2; (C) control scrambled SATB1-siRNA-N; (D) non-transfected controls. Early apoptotic cells were 7-AAD negative and PE-Annexin V positive, and late apoptotic cells were positive for 7-AAD and Annexin V. siRNA, small interfering RNA; SATB1, special AT-rich sequence-binding protein-1; 7-AAD, 7-amino-actinomycin; PE, phycoerythrin.
