Table V.

Univariate Cox regression analysis of OS and DFS in patients with osteosarcoma.

	Disease-free survival		Overall survival
Category	RR (95% CI)	P-value	RR (95% CI)	P-value
Age, years (≥18/<18)	1.151 (0.714–1.714)	0.564	1.172 (0.705–1.705)	0.541
Gender (male/female)	1.105 (0.681–1.681)	0.686	0.951 (0.570–1.570)	0.020
Tumor size, cm (≥5.0/<5.0)	1.912 (1.184–3.184)	0.008	2.326 (1.388–3.388)	0.001
Clinical stage (IB/III/I+IIA)	4.031 (2.377–6.377)	<0.001	4.098 (2.396–7.396)	<0.001
Histological subtype (mixed/fibroblastic/	1.052 (0.870–1.870)	0.603	1.051 (0.858–1.858)	0.630
(Mixed/fibroblastic/chondroblastic/osteoblastic)
Distant metastasis (present/absent)	1.991 (1.216–3.216)	0.006	3.450 (1.999–5.999)	<0.001
Anatomic location (elsewhere/tibia/femur)	2.513 (1.63–3.63)	<0.001	1.539 (0.919–2.919)	0.101
miR-335 expression (negative/positive)	0.310 (0.184–0.184)	<0.001	0.287 (0.163–0.163)	<0.001
Rock1 expression (positive/negative)	2.633 (1.528–4.528)	<0.001	2.887 (1.579–5.579)	0.001
miR-335/Rock1 expression	2.542 (1.553–4.553)	<0.001	2.931 (1.735–4.735)	<0.001
(miR-335-low and Rock1-high/others)

OS, overall survival; DFS, disease free survival; RR, relative risk; CI, confidence interval; miR, microRNA; Rock1, Rho-associated serine-threonine protein kinase.