Table II.
Primers and conditions for the polymerase chain reaction.
| Gene/internal control | Primers | Probes (5′-3′) | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|---|
| IGF-1 | Forward | GTGGATGAGTGCTGCTTC | 58.0 | 134 |
| Reverse | ACTTCCTTCTGGGTCTTGG | |||
| IGF-2 | Forward | TACCGCCATCTCCCTTCTC | 60.0 | 122 |
| Reverse | TCCCTCTGACTGCTCTGTG | |||
| IGF-1R | Forward | TGCCTTGGTCTCCTTGTC | 58.0 | 154 |
| Reverse | TTTCCCTGCTTTGATGGTC | |||
| IGFBP-3 | Forward | TTTCATCTCTCATCTTTTGTCCTC | 60.0 | 77 |
| Reverse | GCCATTCCTCCTTCCTGTTC | |||
| β-actin | Forward | AGTTGCGTTACACCCTTTCTTGAC | 60.0 | 171 |
| Reverse | GCTCGCTCCAACCGACTGC |
IGF, insulin-like growth factor; IGF-1R, IGF-receptor 1; IGFBP, IGF-binding protein; bp, base pair.