Figure 3.

Effects of SIM treatment on HIF-1α expression in B16.F10 melanoma cells. (A) Western blot analysis of HIF-1α expression levels in normoxic and hypoxic B16.F10 cells after 24 h of incubation with 5 µg/ml SIM. (B) Percentages of HIF-1α expression levels in B16.F10 cells after 24 h of incubation with 5 µg/ml SIM compared with its expression in control cells (either normoxic or hypoxic cells). The results represent the mean ± standard deviation of two independent measurements. Unpaired Student's t-test was performed to analyze the effects of SIM on the tumor cell levels of HIF-1α. *P<0.05. Normoxic cells, expression levels of HIF-1α in control cells after 24 h of incubation with culture medium; Normoxic cells + SIM, expression levels of HIF-1α in normoxic cells after 24 h of incubation with 5 µg/ml SIM; Hypoxic cells, expression levels of HIF-1α in control cells after 24 h of incubation with medium supplemented with 200 µM CoCl₂; Hypoxic cells + SIM, expression levels of HIF-1α in hypoxic cells after 24 h of incubation with medium supplemented with 200 µM CoCl₂ and treated with 5 µg/ml SIM; ns, not significant; HIF, hypoxia-inducible factor; SIM, simvastatin.