Figure 4.

Effects of SIM treatment on NF-κB expression in B16.F10 melanoma cells. (A) Western blot analysis of NF-κB expression levels in normoxic and hypoxic B16.F10 cells after 24 h of incubation with SIM. (B) Percentages of NF-κB expression levels in B16.F10 cells after 24 h of incubation with 5 µg/ml SIM compared with its expression levels in control cells (either normoxic or hypoxic cells). The results represent the mean ± standard deviation of two independent measurements. Unpaired Student's t test was performed to analyze the effects of SIM on tumor cell levels of the transcription factor NF-κB. Normoxic cells, expression levels of NF-κB in control cells after 24 h of incubation with culture medium; Normoxic cells + SIM, expression levels of NF-κB in normoxic cells after 24 h of incubation with 5 µg/ml SIM; Hypoxic cells, expression levels of NF-κB in control cells after 24 h of incubation with medium supplemented with 200 µM CoCl₂; Hypoxic cells + SIM, expression levels of NF-κB in hypoxic cells after 24 h of incubation with medium supplemented with 200 µM CoCl₂ and treated with 5 µg/ml SIM; ns, not significant; SIM, simvastatin; NF, nuclear factor.