Table I.

Effects of 5 µg/ml SIM on B16.F10 melanoma cell production of pro-angiogenic/pro-inflammatory proteins under normoxic and hypoxic conditions.

	Percentage of reduction (−) or increase (+) in tumor cell production of proteins involved in tumorangiogenesis/inflammation following SIM treatment, mean ± SD
Pro-angiogenic/pro-inflammatory proteins	SIM	CoCl2 + SIM
Granulocyte-colony stimulating factor	+19±18.12a	−83.19±0.54d
Granulocyte-macrophage-colony stimulating factor	+22.41±13.20a	−74.00±1.09d
Macrophage-colony stimulating factor	+9.27±0.78a	−82.80±0.13d
Insulin growth factor II	+4.60±2.16a	−82.37±0.22d
Interleukin 1α	+18.00±0.01b	−73.90±0.50d
Interleukin 1β	+22.70±0.93c	−72.64±0.51d
Interleukin 6	+13.63±5.36a	−85.52±0.04d
Interleukin 9	+7.92±1.27a	−71.40±0.35d
Interleukin 12 p40	+80.89±11.48d	−83.26±0.73d
Interleukin 13	+6.19±2.86a	−75.42±0.78d
Tumor necrosis factor α	+58.87±29.45c	−83.98±0.17d
Monocyte chemoattractant protein-1	+24.07±3.21c	−82.65±0.14d
Eotaxin	−4.09±3.11a	−47.31±0.86b
Fas ligand	+5.91±17.93a	−74.8±0.19d
Basic fibroblast growth factor	+22.68±7.57a	−85.19±0.06d
Vascular endothelial growth factor	+0.92±23.31a	−72.22±0.66b
Leptin	+8.64±13.19a	−56.23±1.39a
Thrombopoietin	−7.03±11.62a	−80.83±0.32d

The results represent the mean ± SD of two independent measurements.

^aNot significant

^bP<0.05

^cP<0.01

^dP<0.001. SIM, percentages of reduction (−) or increase (+) in protein production in C26 cells incubated with 5 µg/ml SIM for 24 h compared with their normoxic production in untreated cells; CoCl₂ + SIM, percentages of reduction (−) or increase (+) in protein production in C26 cells incubated with 200 µM CoCl₂ and 5 µg/ml SIM for 24 h compared with their production in cells treated only with CoCl₂. SIM, simvastatin; SD, standard deviation.