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. 2017 Mar 23;13(5):3387–3394. doi: 10.3892/ol.2017.5895

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Copyright: © Xu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — TYMS is a direct target gene of miR-330 in vitro. (A) The miR-330 binding site in the 3′UTR of TYMS and the TYMS 3′UTR mutant sequence. (B) miR-330 inhibited PGL3-TYMS-3′UTR Wt luciferase activity in HEK293T cells (P<0.05), whereas PGL3-TYMS-3′UTR Mut revealed no response to miR-330. (C) TYMS mRNA expression levels were not significantly altered following transfection with miR-330 mimics. (D) The TYMS protein was downregulated in miR-330 mimic-transfected HCT116 and HT29 cells compared with the NC groups. *P<0.05 compared with the NC. TYMS, thymidylate synthase; miR-330, microRNA-330; 3′UTR, 3′untraslated region; Wt, wild-type; Mut, mutant; NC, negative control.