Figure 3.

(A) GFP detection indicated that ≥90% of NSCLC cells were effectively infected with virus particles. (B) Western blotting results revealed that overexpressed RKIP significantly suppressed ERK and STAT3 phosphorylation in A549 and NCI-H1299 cells. (C) Transwell invasion assay was performed to assess the invasiveness of NSCLC cells infected with blank lentivirus or RKIP-containing lentivirus. Representative images of invaded cells are shown, and 5 random fields were counted per well (*P<0.05). GFP, green fluorescent protein; NSCLC, non-small cell lung cancer; ERK, extracellular signal-regulated kinase; pERK, phosphorylated extracellular signal-regulated kinase; STAT3, signal transducer and activator of transcription 3; pSTAT3, phosphorylated signal transducer and activator of transcription 3; IL-6, interleukin 6; RKIP, Raf kinase inhibitor protein.