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. 2017 Mar 14;13(5):3247–3252. doi: 10.3892/ol.2017.5863

Figure 3.

Figure 3.

miR-320a negatively regulates IGF-1R expression by directly targeting its 3′UTR. (A) The predicted binding sites of miR-320a on the 3′UTR of IGF-1R and the mutant IGF-1R 3′UTR sequence at the binding site. (B) The analysis of the relative luciferase activity of PGL3-IGF-1R-3′UTR Wt and PGL3-IGF-1R-3′UTR Mut. *P<0.05 compared with NC (C) Relative expression levels of IGF-1R mRNA in H23 and A549 cells transfected with miR-320a mimics or NC. *P<0.05 compared with NC (D) IGF-1R protein expression levels were decreased by miR-320a mimics in H23 and A549 cells. miR-320a, microRNA-320a; IGF-1R, insulin-like growth factor 1 receptor; 3′UTR, 3′-untranslated region; Wt, wild-type; mut, mutant; NC, negative control.