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. 2016 Dec 5;6:2. doi: 10.1038/s41598-016-0005-4

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© The Author(s) 2016

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Time-dependent trends in mutational mechanisms. (a) The frequency of rpoS mutations in isolated mutants is increasing with time. The x-axis shows days after plating. (b) Isolated mutants show a distinct mutation pattern in the crp gene. Over time, there is a steady increase in mutations originating from guanosine (likely 8-oxo-G) whereas the C-deamination associated mutations decrease.