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. 2016 Dec 23;6:22. doi: 10.1038/s41598-016-0030-3

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© The Author(s) 2016

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LIC2 transports NuMA to the spindle pole during mitosis. (A) Representative 3D reconstructions from confocal immunofluorescence images showing spindle poles in mitotic HeLa cells. LP = lower pole, UP = upper pole in LIC2 depleted (mis-oriented) cells. (B) Average NuMA intensity at poles from quantification using 3-dimensional regions of interest drawn around the centrosomes. Error bars are mean +/− SEM from 3 independent experiments, at least 20 metaphase cells per experiment. (C) Ratio of fluorescence intensities (UP/LP) in metaphase cells–54 cells (control), 57 cells (LIC1 depleted) and 55 cells (LIC2 depleted) over a total of 3 experiments. Error bars are mean +/− SEM from 3 independent experiments. (D) Fold reduction in average NuMA levels at the upper pole upon LIC2 depletion in comparison to control cells from the data used for C.