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. 2017 May 4;7:1505. doi: 10.1038/s41598-017-01620-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Aortic tissue visualizations using multiphoton microscopy. (A) En-face perspective of the IEL of the ascending aorta from a WT mouse using multiphoton microscopy. TPEF signal of elastin in green and SHG signal of collagen in red. (B) Three-dimensional rendering of a portion of the tunica media from the ascending aorta of a WT mouse. (C and D) Representative multiphoton images of the tunica media in transverse views of WT (C) and Marfan (D) mice. (E and F) Conventional histological visualization of elastic fibres using Verhoeff-van Gieson staining in WT (E) and Marfan (F) aortic tissue. Scale bar, 10 μm.