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. 2017 May 4;7:1505. doi: 10.1038/s41598-017-01620-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative analysis of density and size of fenestrae seen in IEL and 2^nd elastic laminae from en-face maximal projection images. Fenestrae density (A) and size (B) seen in WT (orange) and Marfan (MF, blue) IEL and 2^nd lamellae. Statistical significance between groups is indicated by asterisks, and defined in the Materials and Methods section. Interquartile boxplots with minimum and maximum whiskers. Forty maximal projections were analysed and a total of 6,400 fenestrae were quantified.