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. 2017 May 4;7:1513. doi: 10.1038/s41598-017-01587-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical staining of phosphorylated α-synuclein in the temporal lobe of the GRN mutation cases. Phosphorylated α-synuclein -positive structures were observed in the inferior temporal cortex (A), superior temporal cortex (B) and tuberomammillary nucleus (C) in case 4, and in the temporal cortex in case 1 (D), case 2 (E) and case 3 (F). Sections were counterstained with Kernechtrot stain solution. The scale bar in (A) applies to (C–F) (200 μm). The scale bars in (B), in insert (A,C and D–F) are 50 μm.