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. 2017 May 4;7:1506. doi: 10.1038/s41598-017-01698-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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S.aureus biofilm was grown on glass beads and incubated with 133–533 µg/ml AMP2 and heat flow of the bacteria was measured over 48 hours. (A) Total heat over time. red: growth control (GC), gray: negative control (NC) - beads without bacteria, green: 133 µg/ml AMP2, purple: 267 µg/ml AMP2, black: 533 µg/ml µg/ml AMP2 (n = 6). Kruskal-Wallis, Mann-Whitney, Bonferroni-Holm post-hoc test: *Significant compared to growth control, p ≤ 0.05. (B) After calorimetry measurement, samples and glass beads were plated on one half of a CA plate, respectively. Shown are exemplarily plates with medium sample on the left and three glas beads (circles) of 133, 267, 533 µg/ml AMP2 and 128 µg/ml daptomycin on the right side.