Table 3.
All primer pairs were designed with the Primer3web web page and intron spanning.
| Gene | Name | Efficiency | forward (F) and reverse (R) sequence 3′-5′ |
|---|---|---|---|
| 18 S | 18 S | 1.79 | F: CGG AAA ATA GCC TTT GCC ATC |
| R: AGT TCT CCC GCC CTC TTG GT | |||
| alkaline phosphatase | AP | 1.81 | F: GGA AAT CTG TGG GCA TTG TG |
| R: CCC TGA TGT TAT GCA TGA GC | |||
| collagen type I, alpha 1 | Col1 | 1.92 | F: TGA CCT CAA GAT GTG CCA CT |
| R: ACC AGA CAT GCC TCT TGT CC | |||
| runt-related transcription factor 2 | RUNX2 | 1.86 | F: GCC CCC AAA CAG TAT CTT GA |
| R: GCC TGA AGT GAG GTT TTA GGC |
Using the OligoAnalyzer 3.1 software all primer pairs were tested for self-dimers, hetero-dimers or hairpin production. The amplicon size was chosen between 150–250 base pairs.