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. 2017 May 4;7:1498. doi: 10.1038/s41598-017-01659-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Treatment with cryptolepine reduces the protein levels of mitochondrial dynamics and decreases ATP levels. A375 and Hs294t melanoma cells were treated with cryptolepine (0, 2.5, 5.0 and 7.5 µM) for 24 h. (a) Cell lysates were prepared and subjected to analysis of mitochondrial fission and fusion proteins. Equal loading of proteins on the gels was verified by stripping the membrane and reprobing with β-actin. (b) ATP production was determined in cell lysates using an ATP determination kit, as described in Material and Methods. The experiment was performed in triplicate and results are expressed as mean ± SD in terms of percentage of control. Statistical significance versus control, *P < 0.05, ^¶ P < 0.01 and ^† P < 0.001.